Brown nitrogen fixing bacteria|337393 |BNCC

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Azotobacter vinelandii

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	30 ℃; 18-24h; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 0.5-1mm, round, with neat edges, opaque, yellow on the front, light color, raised in the middle, smooth on the surface, moist texture, G - (red), large oval shaped rod-shaped bacteria, purity: pure
Sharing mode	Public welfare sharing

1.Description

1.Name: Azotobactervinelandii

2.BNCC No.:337393

3.Biosafety level: 4

2.Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1, Tryptone soybean agar (TSA): tryptone (casein trypsin digest) 15.0g, soybean peptone (soybean meal papain digest) 5.0g, sodium chloride 5.0g, agar 15.0g,pH7.3±0.2. 121 ℃,15min.

2.Atmosphere: aerobic

3.Temperature: 30 ℃

4.Notes:

1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and long-term storage is not recommended.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Users should ensure the safe storage and operation of the strain.Waste generated from the handling process should be discarded after high-pressure sterilization.

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