Thermophilic thermophilic bacteria|186418 |BNCC

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Thermus thermophilus

Subculture procedure	① Prepare 1 bottle of liquid medium, about 500mL; ② Disinfect the surface of the ampoule tube, open it in a safe cabinet, burn the top with an alcohol lamp, quickly add sterile water to the burning area to break it, and then use tweezers to knock it open; ③ Transfer approximately 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid culture medium; ④ Place the liquid test tube under the specified "culture conditions" mentioned above for cultivation. If the bacterial liquid is turbid or there is a large amount of bacterial growth at the bottom, it can be used.
Growth conditions	75 ℃; 24-48 hours; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Liquid medium turbid, G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing

Thermus thermophilus

Storage conditions: 2~8 ℃

No. 186418

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1,handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. The freeze dried culture shall be used up once and shall not be retained. Handling instructions of suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 75℃, aerobic, Thermus thermophilus medium, 24-48h. Thermus thermophilus medium: yeast powder 4.0g; polypeptone 8.0g; NaCl 2.0g; agar 20g; distilled water 1L. Adjust pH to 7.0. Sterilize at 121°C for 15min.

Recovery steps:

① Prepare a φ18mm test tube of 10ml liquid medium.

② Sterilizing the ampoule, open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

③ Draw 0.5mL of liquid culture medium into the freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

④ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 24h the liquid medium bacterial fluid is turbid, the plate strain is weak.
colony morphology: (above)	thermophilic thermophilic bacteria are gram-negative bacteria. thermophilic thermophilic bacteria medium liquid culture medium is turbid, there is a large amount of precipitation at the bottom of the test tube, and the growth of plate strains is weak. Liquid culture is recommended.
Conclusion:	good viability, no abnormal colony morphology, qualified

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