Porphyromonas gingivalis|337441 |BNCC

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Porphyromonas gingivalis

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 7-10 days; anaerobic
Subculture procedure	① Prepare 1-2 agar plates as described above (pre-conditioned in an anaerobic environment for 24 h for oxygen removal).② In a biosafety cabinet, open the vial and briefly flame the top with an alcohol burner, then immediately add sterile water to break the seal. Use sterile forceps to crush the vial.③ Aspirate 0.5 mL of sterile water (pre-conditioned in an anaerobic environment for 24 h for oxygen removal) into the freeze‑dried vial, thoroughly dissolve the bacterial powder, then transfer 200 μL of the suspension onto each Agar plate and spread evenly.④ Incubate the plates under the specified culture conditions. The strain is ready for use once growth appears.
Storage conditions	2-8 ℃
Safety level	2
morphology	The colonies measure 1-2 mm in diameter, are circular with regular margins, and are opaque. They exhibit a black pigmentation on the surface, have a raised center, and are smooth and moist in texture, making them easy to lift. The organisms are Gram-negative (G⁻, appearing red) and short rod-shaped (short bacilli).
Sharing mode	Public welfare sharing