Porphyromonas gingivalis|337441 |BNCC

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Porphyromonas gingivalis

Subculture procedure	① Prepare 1-2 agar plates as described above (pre-conditioned in an anaerobic environment for 24 h for oxygen removal).② In a biosafety cabinet, open the vial and briefly flame the top with an alcohol burner, then immediately add sterile water to break the seal. Use sterile forceps to crush the vial.③ Aspirate 0.5 mL of sterile water (pre-conditioned in an anaerobic environment for 24 h for oxygen removal) into the freeze‑dried vial, thoroughly dissolve the bacterial powder, then transfer 200 μL of the suspension onto each Agar plate and spread evenly.④ Incubate the plates under the specified culture conditions. The strain is ready for use once growth appears.
Growth conditions	37 ℃; 7-10 days; anaerobic
Storage conditions	2-8 ℃
Safety level	2
morphology	The colonies measure 1-2 mm in diameter, are circular with regular margins, and are opaque. They exhibit a black pigmentation on the surface, have a raised center, and are smooth and moist in texture, making them easy to lift. The organisms are Gram-negative (G⁻, appearing red) and short rod-shaped (short bacilli).
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Porphyromonas gingivalis

Storage conditions : 2~8 ℃

No. : 337441

Product format: freeze dried, 200ul

Validity period: 6 years

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :37 ℃, anaerobic, Columbia blood plate (ready-to-use type),7-10d.

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4)Put the plates under the above culture conditions for cultivation for 7-10days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability	good viability,in 7-10d liquid medium become turbid, obvious strain layer occurs on the plate,colony is typical on marked plate.
colony morphology: (above)	size: 1-2mm shape: round edge: neat transparency: opaque color: white uplift: middle raised surface: bright and smooth texture: moist and viscous
conclusion	good viability, no abnormal colony morphology, qualified

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