Enteral invasive Escherichia coli|186731 |BNCC

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Escherichia coli EIEC

Subculture procedure	① Prepare the above 2 plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take about 0.5mL of sterile water and pour it into the freeze-drying tube. After fully dissolving the bacterial powder, pour it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the agar plate under the above cultivation conditions for cultivation.
Growth conditions	37 ℃; 18-24h; aerobic;
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, milky white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing

1. Description

1. Name: Escherichia coli EIEC

2. BNCC No.: 186731

3. Biosafety level: 3

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

3. Growth Conditions:

1. Nutritious gravy agar: beef paste 5g, peptone 10g,NaCl 5g, agar 20.0g, distilled water 1.0L,pH 7.0. 121 ℃,15 minutes sterilization. [Note] Add 5mg MnSO4·H2O when culturing Bacillus,which is beneficial to spore production.

2. Atmosphere: aerobic

3. Temperature: 37 ℃

4. Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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