Salt tolerant Aspergillus|187704 |BNCC

Essential Information Certificate Related Products

Cladosporium halotolerans

Subculture procedure	① Prepare 1 test tube containing 5-10 mL of liquid culture medium and 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	28 ℃; 5-7 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	The colonies exhibit a velvety texture and develop irregular concentric rings on the surface. The margins are uneven, and the colonies appear dark green to deep green in color. The reverse side shows a faint yellowish-white hue, with no exudate observed.b
Sharing mode	Public welfare sharing

Cladosporium herbarum

Description

1. BNCC No.: 187704

2. Biosafety level: 4

Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

Growth Conditions:

1. Wort agar medium: malt paste powder 130g, agar 15g, chloramphenicol 0.1g, distilled water 1L,pH6.0±0.2.

2. Atmosphere: aerobic

3. Temperature: 28-30 ℃

Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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