Streptococcus agalactiae|188152 |BNCC

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Streptococcus agalactiae-BNCC
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  • Streptococcus gallolyticus-BNCC

Streptococcus gallolyticus

  • Price： Contact
  • number：188152
  • Form：
    The colony diameter is 0.5-1mm, circular, with irregular edges, opaque, pale yellow on the front and back, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), cocci, purity: pure
Standard strain Quantitative strain DNA extraction
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Streptococcus gallolyticus
Subculture procedure ① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions 30 ℃; 24-48 hours; aerobic
Storage conditions 2-8 ℃
Safety level 1
morphology The colony diameter is 0.5-1mm, circular, with irregular edges, opaque, pale yellow on the front and back, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), cocci, purity: pure
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Streptococcus gallolyticus

BNCC No.: 188152

Biosafety level:  4

Storage conditions: Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

Growth Conditions: 

1. Tomato juice culture medium: yeast extract 7.5g, peptone 7.5g, glucose 10.0g,K2HPO4 2.0g,CaCO3 10.0g, tomato juice 100.0ml, twain 80 0.05-0.5ml, agar 25.0g, distilled water 900.0ml,pH7.0. [Note] Squeeze to prepare tomato juice, pH7.0.

2. Atmosphere: aerobic

3. Temperature:  28-30 ℃

4. Notes: 

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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