Fusarium oxysporum|337554 |BNCC

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Fusarium solani

Subculture procedure	① Dissolve the frozen tube in a water bath at 30 ℃ and shake quickly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates; ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	28 ℃; 5-7 days; aerobic
Storage conditions	-80 ℃
Safety level	1
morphology	Small filamentous fungi, with obvious colonies on the comprehensive PDA medium, white and dense colonies, spreading and growing in concentric circles towards the edge of the Agar plate, and yellow colonies on the back of the medium
Sharing mode	Public welfare sharing

Fusarium solani

storage conditions: 2~8 ℃

number: 337554

product format: agar slant in 14mm test tube

Validity: 30 days

Biosafety level: 1, handle in ultra-clean table or safety cabinet

Receiving notice:if any abnormality is found on the day of receiving goods, please contact customer service within 24 hours. if the time is overdue, it will be regarded as good receiving goods. The agar slant can be used directly. in principle, it can be used many times without contamination within the storage period, but its vitality will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement service will not be provided if the strain is abnormal and inactivated.

culture conditions: 25-28 ℃, aerobic, integrated PDA,5d. Comprehensive PDA: potato boil 1.0L, glucose 20.0g,KH₂PO₄3.0g,MgSO₄·7H₂O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0 ±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.

Recovery steps:

1 prepare 1-2 pieces of PDA plates;

2 sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

3 cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;

4 lay flat the small pieces to the center of the agar plate;

5 put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
Viability:	good viability. in 5 days, plate colony is obvious
colony morphology:	Small filamentous fungi have obvious colonies on the integrated PDA medium, white and dense colonies, spreading and growing concentrically to the edge of the plate, and the back of the medium is yellow.
Conclusion:	good viability, no abnormal colony morphology, qualified

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