Fine yellow chain fungi|337579 |BNCC

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Streptomyces microflavus

Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the tablet, open it in the safety cabinet; ③ Take 1-3ml of sterile water and pour it into the Agar plate. Use a sterile coating rod to scrape the bacterial coating back and forth on the surface of the Agar plate to make a bacterial suspension; ④ Suck the bacterial suspension with a pipette, pour it onto a fresh Agar plate, and apply evenly; ⑤ Place the agar plate under the above cultivation conditions for cultivation.
Growth conditions	28 ℃; 3-5 days; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, white on the front, light in color, raised in the middle, rough on the surface, bright on the surface, hard in texture, difficult to pick/cut, does not produce pigments, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing

1.Description

1. Name:Streptomyces microflavus

2. BNCC No.:337579

3. Biosafety level: 4

2. Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth conditions

1. Gao's synthetic agar no 1: KNO3 1g, soluble starch 20g ,K2HPO4 0.5g, MgSO4.7H2O 0.5g ,NaCl 0.5g ,FeSO4 0.01g, agar 20g, water 1000ml, pH 7.2-7.4

2. Atmosphere: aerobic

3. Temperature: 28 ℃

4.Notes:

1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and long-term storage is not recommended.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Users should ensure the safe storage and operation of the strain.Waste generated from the handling process should be discarded after high-pressure sterilization.

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