Aspergillus fumigatus|338385 |BNCC

BeNa Culture Collection

Aspergillus fumigatus-BNCC
Aspergillus fumigatus-BNCC
Aspergillus fumigatus-BNCC
  • BNCC
  • Aspergillus fumigatus Fresenius-BNCC
  • Aspergillus fumigatus Fresenius-BNCC
  • Aspergillus fumigatus Fresenius-BNCC

Aspergillus fumigatus Fresenius

  • Price: Contact
  • number:338385
  • Form:
    Spread and grow, producing gray green spores
Standard strain Quantitative strain DNA extraction
Package:
Essential Information Certificate Related Products
Aspergillus fumigatus Fresenius
Subculture procedure ① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and pour it into the freeze-drying tube. After fully dissolving, mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used.
Growth conditions 28 ℃; 5-7 days; aerobic
Storage conditions 2-8 ℃
Safety level 2
morphology Spread and grow, producing gray green spores
Sharing mode Public welfare sharing

Aspergillus fumigatus Fresenius

Storage conditions: 2~8 ℃

No.: 338385

Product format: freeze dried, 200ul

Validity period: 6 years

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Growth conditions:28 ℃, aerobic, 5-7 days, Chastler agar, Chastler agar: 3g sodium nitrate, 1g dipotassium hydrogen phosphate, 0.5g magnesium sulfate, 0.5g potassium chloride, 0.01g ferrous sulfate, 30g sucrose, 15g agar, 1.0L distilled water, pH 6.2±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4)Put the plates under the above culture conditions for cultivation for 5-7days.

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

   

item test result
viability good viability, in 5-7 days strain layer become obvious,  colony is typical
colony morphology: (above) filamentous fungi, with obvious colonies on Cha's agar medium, white hyphae, sparse and long, hyphae spread and grow, producing a large number of gray spores.
conclusion good viability, no abnormal colony morphology, qualified
Download certificate
Please set your password: