Difficult Clostridium difficile|340237 |BNCC

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Clostridioides difficile

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 48-72h; anaerobic;
Subculture procedure	① Prepare one sterile water and one Agar plate (pre placed in an anaerobic environment for 24 hours of deoxygenation) with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 200 μ L of sterile water and pour it into a freeze-drying tube. After fully dissolving the bacterial powder, pour it into a Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony has a diameter of 1-2mm, irregular shape, irregular edges, opacity, gray white front, flat shape, smooth surface, moist texture, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing