BeNa Culture Collection
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| Culture medium | BNCC360816 |
| Description | Fan's culture medium |
| Composition | K2HPO4:1.0g, MgSO4:0.5g, Yeast Extract:10.0g, Agar:20.0g, pH:7.0-7.2(25℃) |
| Growth conditions | 30 ℃; 5-7 days; 40W tungsten filament light for anaerobic treatment; |
| Subculture procedure | ① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 0.5-1mm, circular, with irregular edges, dark brown color, raised in the middle, smooth surface, bright surface, moist texture, easy to pick up, G - (red), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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