Sclerotium griseum|118626 |BNCC

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Aureobasidium pullulans (de Bary) G. Arnaud

Subculture procedure	① Prepare 1 test tube containing 5-10 mL liquid medium or sterile water and 2 agar plates; ② Open in a safety cabinet, cauterize the top with a spirit lamp, then quickly add sterile water to rupture it; break it apart with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve thoroughly, then return to the liquid test tube and mix well; ④ Pipette 0.2mL bacterial suspension onto plates, spread evenly; repeat twice to obtain two plates; ⑤ Incubate under the specified conditions. The culture is ready for use once the strain grows.
Growth conditions	25-28°C; 3-5 days; aerobic
Storage conditions	2-8°C
Safety level	1
morphology	Slowly spreading growth, circular, irregular edges, opaque, gray on the front, dark yellow on the back, flat morphology, dry texture
Sharing mode	Public welfare sharing

Aureobasidium pullulans

Storage conditions: 2~8 ℃

No. 118626

Product format : freeze dried,200ul

Validity period: 24 months

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :25-28 ℃, aerobic, PDA. PDA: potato extract 6g, glucose 20g, agar 20g,pH natural, 121 ℃, 15min.

Recovery steps:

(1)Prepare 2 pieces of PDA plates or 2 agar slants;

(2)Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate; or transfer appropriate solution to the agar slant.

(4)Place the plates uprightly and agar slant obliquely under the above culture conditions for 5-7 days. Or contact our technicians for the inoculation of agar slant - filamentous fungi.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 5 days plate colony is obvious
colony morphology:	The morphology of bacteria is obviously visible on PDA agar medium, forming fungal colonies with loose edges and irregular edges, and the colonies are dark green.
Conclusion:	good viability, no abnormal colony morphology, completely consistent with the above figure, qualified

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