BeNa Culture Collection
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| Subculture procedure | ① Prepare one 18mm test tube of liquid medium, approximately 10mL (placed in anaerobic environment for 24 hours of deoxygenation in advance); ② Disinfect the surface of the ampoule tube, open it in a safe cabinet, burn the top with an alcohol lamp, quickly add sterile water to the burning area to break it, and then use tweezers to knock it open; ③ Transfer approximately 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back to the liquid culture medium in the test tube; ④ Place the liquid test tube under the specified "culture conditions" mentioned above for cultivation. If the bacterial liquid is turbid or there is a large amount of bacterial growth at the bottom, it can be used. |
| Growth conditions | 37 ℃; 24-48 hours; anaerobic |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | G - (red), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
Fusobacterium nucleatum subsp.
Storage conditions : 2~8 ℃
No. : 337608
Product format: freeze dried, 200ul
Validity period: 6 years
Biosafety level : 2 , handle in safety cabinet
Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.
Growth conditions :37°C, anaerobic, thioglycolate liquid medium, 3-4 days. Thioglycolate liquid medium: caseptone (trypsin hydrolysis) 15.0g, yeast extract 5.0g, glucose 5.0g, sodium thioglycolate 0.5g,L-cystine 0.5g, sodium chloride 2.5g, azure 0.001g, agar 0.75g, distilled water 1.0L,pH 7.1±0.2. Sterilization at 121 ℃ for 15min.
Recovery steps:
(1)Prepare a φ18mm test tube of 10ml liquid medium(put in an anaerobic environment for deoxygenation in advance for 24 hours);
(2)Sterilizing the ampoule, open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3)Draw 0.5mL of liquid culture medium into the freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4)Put the test tube under the above culture conditions for cultivation. The culture solution is obviously turbid or mass growth at the solution bottom occur, this indicates the bacterial grows well.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:
| item | test result |
| viability | good viability,in 3-4 days liquid medium become turbid |
| colony morphology: (above) | Fusobacterium nuclei with nuclear subspecies, microscopic examination shows that gram-negative bacteria have slender morphology, sharp ends are like shuttle, thioglycolate liquid culture medium is turbid, liquid culture is recommended to use liquid culture |
| conclusion | good viability, no abnormal colony morphology, qualified |
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