Bacillus subtilis|342457 |BNCC

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Parabacteroides goldsteinii

Subculture procedure	① Prepare 1-2 fresh agar plates as specified above (pre-conditioned in an anaerobic environment for 24 h to remove oxygen).② Working in a biosafety cabinet, open the vial and briefly flame the top with an alcohol burner. Immediately add sterile water to break the seal, then crush the vial using sterile forceps.③ Aspirate 0.5 mL of liquid medium or sterile water (pre-conditioned in an anaerobic environment for 24 h to remove oxygen) into the freeze‑dried vial, thoroughly dissolve the contents, and mix well.④ Transfer 0.2 mL of the resulting bacterial suspension onto an agar plate, spread evenly, and repeat to inoculate a second Agar plate.⑤ Incubate the plates under the designated culture conditions. The strain is ready for use once growth appears.
Growth conditions	37 ℃; 3-4 days; anaerobic;
Storage conditions	2-8 ℃
Safety level	2
morphology	Size: 1-2 mm，Shape: Circular，Margin: Irregular，Transparency: Opaque，Color: Grayish-white，Elevation: Raised center，Surface: Glossy and smooth，Texture: Moist and easy to lift，The colonies exhibit an irregular margin with a grayish-white surface that appears darker in color. They are smooth, glossy, and moist in texture, and produce no pigment. The organisms are Gram-negative (G⁻, appearing red) and rod-shaped (bacilli). Purity: pure.
Sharing mode	Public welfare sharing

Parabacteroides goldsteinii

Storage conditions: 2~8 ℃

No. 342457

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 2, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, anaerobic, Colombian blood plate (ready-to-use type),3-4 days.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates;

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5ml of sterile water （place in anaerobic environment for 24hours before usage）into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 3-4 days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 4 days strain layer become obvious, colony is typical
colony morphology: (above)	Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque Color: off-white uplift: middle convex surface: bright and smooth texture: moist and easy to stir
Conclusion	good viability, no abnormal colony morphology, qualified

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