Staphylococcus aureus|351955 |BNCC

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Staphylococcus aureus

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	37 ℃; 18-24h; aerobic;
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 1-2mm, circular, with neat edges, golden yellow on the front and yellow on the back, raised in the middle, smooth and bright on the surface, moist texture, no pigment production, G+(blue purple), cocci, purity: pure
Sharing mode	Public welfare sharing

Staphylococcus aureus

Storage conditions : -20 ℃

Validity period: 24 months

No. : 351955

Product format: freeze dried, 200ul

Biosafety level :2,handle in safety cabinet

Usage : pharmacopoeia, GB 4789, control quality control strain

Medium: BNCC Medium 1: nutrition gravy medium beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH7.0. Sterilization at 121 ℃ for 15min. [Note] Add 5mg MnSO4·H2 when culturing Bacillus,which is beneficial to spore production.

Growth conditions temperature: 37 ℃ Gas environment: aerobic

Recovery steps:
(1)Prepare a sterile test tube of 10ml liquid medium and 2 pieces of 90mm agar plates;
(2)Sterilizing of the ampoule, under aseptic conditions, remove the cap and take out the inner vial. lift the cotton plug, rehydrate the pellets by drawing 0.5ml of liquid culture medium into the vial.
(3)Fully dissolved, transfer the solution to the liquid test tube, mix evenly;
(4)Inoculate a plate with the solution, distribute it well in 200ul/ plate; the plates are not allowed to seal by wrapping.
(5)Put all the plates and test tubes under the above culture conditions for cultivation, The culture solution is obviously turbid after 18-24 hours, and this indicates the bacterial grow well.

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