BCG pMV361 eGFP fluorescently labeled strain|391132 |BNCC

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BCG-pMV361-eGFP

Culture medium	BNCC393078
Description	Middlebrook 7H9 Agar Plate with 25μg/mL Kanamycin (Ready-to-use)
Composition	(NH₄)₂SO₄:0.5g, KH₂PO₄:1.0g, Na₂HPO₄:2.5g, Na₃C₆H₅O₇:0.1g, MgSO₄:0.05g, CaCl₂:0.0005g, ZnSO₄:0.001g, CuSO₄:0.001g, L-Glutamic Acid(C₅H₉NO₄):0.5g, C₆H₈FeNO₇:0.04g, Vitamin B6(C₈H₁₁NO₃.HCl):0.001g, Biotin(C₁₀H₁₆N₂O₃S):0.0005g, ADC Supplement:10%(added at 50℃), Agar:18.0g, pH:6.6±0.2(25℃)
Growth conditions	37 ℃; 2-3 weeks; aerobic;
Subculture procedure	① Dissolve the Frozen vials in a 37 ℃ water bath and shake rapidly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μ L per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation. During the cultivation process, the agar plate needs to be kept moist, and 7H10 broth can be added.
Storage conditions	-80 ℃
Safety level	2
morphology	Green fluorescence
Sharing mode	Public welfare sharing