Streptococcus thermophilus|186558 |BNCC

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Streptococcus thermophilus

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	37 ℃; 2-3 days; anaerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	MRS liquid culture appears turbid with substantial sediment at the bottom of the tube. The growth of the strain on agar plates is weak, and it is recommended to use liquid culture for cultivation.
Sharing mode	Public welfare sharing

Streptococcus thermophilus

Storage conditions : 2~8 ℃

No. 186558

Product format : freeze dried,200ul

Validity period: 6 years

Biosafety level : 1

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. The freeze dried culture shall be used up once and shall not be retained. Handling instructions of suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :37 ℃, anaerobic, MRS medium, 18-24h. MRS medium: peptone 10.0g, beef paste 10.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.04g, Tween 80 1.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L. pH 5.7±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1) Prepare a φ18mm sterile test tube of 25ml liquid medium; Silica gel plug is used for the liquid test tube;

(2) Sterilize the ampoule, use a small grinding wheel to make a circle at 1/2 of the tube body under strict aseptic conditions,, and then break it

(3) Draw 0.5ml of liquid growth medium into a freeze dried ampoule, make it full dissolved;

(4) Transfer all the solution to the liquid test tube, plug the tube with silica gel.

(5) Place the liquid test tube in incubator under the above growth conditions for cultivation, The culture solution is obviously turbid or precipitated, and this indicates the bacterial grows well.

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