Morchella genus|190541 |BNCC

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Morchella sp.

Subculture procedure	① Prepare 1-2 plates as mentioned above; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the Agar plate and onto the surface of the agar; ⑤ Cultivate the flat Agar plate under the above cultivation conditions, and the strain can be used once it grows.
Growth conditions	28 ℃; 5-7 days; aerobic
Storage conditions	-80℃
Safety level	1
morphology	A small filamentous fungus cultured on comprehensive PDA medium exhibits distinct colonies. The mycelium appears grayish-white, sparse, and low-profile, spreading towards the edges of the Agar plate. The reverse side of the Agar plate shows a pale yellow coloration.
Sharing mode	Public welfare sharing

Description

1. Name: Morchella sp.

2. BNCC No.: 190541

3. Biosafety level: 4

Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

Growth Conditions

1. Medium components: potato extract 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4.7H2O 1.5g, vitamin B1 trace, agar 15.0g,pH6.0. 121 ℃,15 minutes sterilization.20% potato liquid configuration: take 200g of peeled potato, boil for 30min, filter to take filtrate, and make up to 1.0l.

2. Atmosphere: aerobic

3. Temperature: 25 ℃

Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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