BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC352232 |
| Description | Tryptic Soy Broth |
| Composition | Tryptone:17.0g, Soy Peptone:3.0g, NaCl:5.0g, K2HPO4:2.5g, Glucose:2.5g, pH:7.3±0.2(25℃) |
| Growth conditions | 30°C; 18-24h; aerobic |
| Subculture procedure | ① Prepare two plates as above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then promptly add sterile water to rupture. Crush ampoule with forceps; ③ Pipette 0.5 mL sterile water into lyophilized tube. Thoroughly dissolve bacterial powder, then inoculate two plates (200 μL/Agar plate) with uniform spread; ④ Incubate plates under specified conditions until colonies emerge. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 1-2 mm; irregular shape with jagged edges; opaque, pale yellow surface; flat morphology; rough texture; dry appearance; Gram-positive (blue-violet staining); bacilli |
| Sharing mode | Public welfare sharing |
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