Clostridium|195293 |BNCC

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Clostridium sp

Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the tablet, open it in the safety cabinet; ③ Suck 1-3 ml of sterile water (placed in an anaerobic environment for 24 hours) and pour it into a Agar plate. Use a sterile coating rod to scrape the bacterial moss back and forth on the surface of the Agar plate to make a bacterial suspension; ④ Suck the bacterial suspension with a pipette, pour it onto a fresh Agar plate, and apply evenly; ⑤ Place the agar plate under the above cultivation conditions for cultivation.
Growth conditions	37 ℃; 24-48 hours; anaerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Colony diameter: 1-2 mm; circular, irregularly margined, opaque; upper surface grayish-white, centrally convex, smooth, glossy, moist texture, easily lifted; G+ (blue-violet), bacilli; purity: pure
Sharing mode	Public welfare sharing

1.Description

1. Name：Clostridium kluyveri

2. BNCC No.：195293

3. Biosafety level：4

2.Storage conditions：

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. Thioglycolate medium: caseptone 15.0g, yeast leaching powder 5.0g, glucose 5.0g, sodium thioglycolate 0.5g,L-cystine 0.5g, azure 0.001g, sodium chloride 2.5g,pH7.1±0.2. 121 ℃,15min.

2. Atmosphere：anaerobic

3. Temperature：37 ℃

4.Notes：

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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