Oriental Isa Yeast|225398 |BNCC

Essential Information Certificate Related Products

lssatchenkia orientalis

Subculture procedure	① Prepare 1-2 YM plates; ② Disinfect the surface of the ampoule. Open it in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it. Crush it with forceps; ③ Pipette 0.5mL sterile water into the freeze-dried vial. After complete dissolution, directly pipette the suspension (200μl/vial) onto YM plates and spread evenly; ④ Incubate plates under the specified conditions for 24–48 hours.
Growth conditions	30°C, 24-48h, aerobic
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1–2 mm; circular shape with regular margins; opaque, creamy-white upper surface; centrally convex; glossy surface; moist texture; Gram-positive (blue-violet); oval shape
Sharing mode	Public welfare sharing

lssatchenkia orientalis

BNCC No.：225398

Storage conditions: 2~8 ℃

Product format： freeze dried, 200ul

Validity period: freezed-dried tube 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:25-28 ℃, aerobic, YM medium. YM medium: yeast extract, 3.0g malt extract, 3.0g glucose, 10.0g protein block, 5.0g agar, 20.0g distilled water 1000 ml ,pH 6.2 +/- 0.2,121 ℃,15min.

Recovery steps:

(1) Prepare 1-2 YM plates;

(2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) Put the agar plates under the above culture conditions for cultivation 24-48 hours.

recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability,in 40h YM plate marking colony is obvious
colony morphology:	size: medium color: milky white shape: round edge: neat edge wet and dry: wet and smooth: smooth transparency: opaque uplift: uplift
conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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