Escherichia coli|276498 |BNCC

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Escherichia coli (Migula) Castellani and Chalmers

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37°C, 18-24h, aerobic
Subculture procedure	① Prepare 1 test tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to break it; subsequently crush it with forceps; ③ Pipette 0.5mL liquid medium into the lyophilized tube. After complete dissolution, return the solution to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid test tubes and plates under the specified conditions. The culture is ready for use once growth appears.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; circular, with neat edges; opaque, grayish-white upper surface; flat morphology; smooth, shiny surface; moist texture; easily liftable; G- (red); bacillus; purity: pure
Sharing mode	Public welfare sharing