Pseudomonas aeruginosa|358318 |BNCC

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Pseudomonas aeruginosa

Subculture procedure	① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	37 ℃; 18-24h; aerobic;
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 1-2mm, circular, with irregular edges, opaque, gray white front, flat shape, rough surface, gray surface, dry texture, easy to pick up, G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing

Pseudomonas aeruginosa

Storage conditions : 2~8 ℃

No. : 358318

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 2, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receiving goods, please contact customer service within 24 hours in time. if it is overdue, it will be deemed as good receipt. Dry powder shall not be retained after one use, and it can be used after being activated for 1~2 generations. please refer to the attached page for the use of the flat panel. Please activate in strict accordance with this instruction, otherwise the replacement service will not be provided if the strain is abnormal and inactivated.

Growth conditions: 37 ℃, aerobic, Colombian blood plate (ready-to-use type),18-24h.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates;

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 18-24 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows

Item	test results
viability:	good viability ，in 24 hours strain layer is obvious;colony is typical on the streaked plate
Colony morphology: (pictured above)	Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque Color: off-white Protuberance: Middle convex surface: bright and smooth texture: moist and easy to stir
conclusion: good viability, no abnormal colony morphology, qualified

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