Multi branch cordyceps|150056 |BNCC

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Cordyceps polyarthra

Subculture procedure	① Prepare 1-2 tablets mentioned above; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the Agar plate and onto the surface of the agar; ⑤ Cultivate the flat Agar plate under the above cultivation conditions, and the strain can be used once it grows.
Growth conditions	28 ℃; 5-7 days; aerobic;
Storage conditions	-80℃
Safety level	1
morphology	Spread growth, filamentous, irregular edges, opaque, white front, yellow back, light color, purity: pure
Sharing mode	Public welfare sharing

Cordyceps polyarthra

Storage conditions : 2~8 ℃

No. : 150056

Product format :agar slant in 14mm test tube

Validity : growing culture, in 30 days

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28 ℃, aerobic, corn flour medium, 3-5 days. Corn flour medium: corn flour 5.0g, peptone 0.1g, glucose 1.0g, agar 13.0g, distilled water 1.0 L, natural pH. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm²;

(4) lay flat the small pieces to the center of the agar plate;

(5)put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 5days strain layer become obvious
colony morphology:	small filamentous fungi, with obvious colonies on corn flour medium, white, sparse hyphae, almost no spread, and light yellow on the back of the medium.
Conclusion:	good viability, no abnormal colony morphology, qualified

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