BeNa Culture Collection
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| Culture medium | BNCC354756 |
| Description | Improved MRS culture medium |
| Composition | Peptone:10.0g, Beef Extract:10.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO4:0.2g, CH3COONa:5.0g, C6H8O7.(NH4)3:2.0g, K2HPO4:2.0g, MnSO4:0.05g, Tween 80:1.0g, L-Cysteine:0.5g, Agar:20.0g, pH:6.2±0.2(25℃) |
| Growth conditions | 30 ℃, 48-72 hours, facultative anaerobic |
| Subculture procedure | ① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and pour it into the freeze-drying tube. After fully dissolving, transfer it back to the liquid test tube and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 1-2mm, circular, with neat edges, opaque, milky white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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