Clostridium perfringens|186499 |BNCC

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Clostridium pasteurianum

Subculture procedure	① Prepare 1-2 plates as above (pre-deoxygenated in anaerobic environment for 24h); ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then rapidly add sterile water to rupture. Crush contents with forceps; ③ Pipette 0.5mL sterile water into lyophilized tube. Fully dissolve powder, then dispense 200μL/well onto plates, spreading evenly; ④ Incubate plates under the specified conditions.
Growth conditions	37°C; 48-72h; anaerobic
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; circular, irregularly margined, opaque; upper surface grayish-white with central elevation; smooth surface, easily liftable; Gram-positive (blue-violet); bacilli; purity: pure
Sharing mode	Public welfare sharing

Clostridium pasteurianum

Storage conditions: 2~8 ℃

No. 186499

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 37℃, anaerobic, Columbia blood plate (ready to use), 48-72h.

Recovery steps:

①Prepare 1-2 of above mentioned plates(Place it in an anaerobic environment for deoxygenation for 24h in advance);

②Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

③Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

④Put the plates under the above culture conditions for cultivation.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 72hours strain layer become obvious;colony is typical on the streaked plate
colony morphology: (above)	size: 1-2mm ; Shape: round ; Edge: irregular ; Transparency: opaque color: off-white ; Protuberance: middle bulge ; Surface: bright and smooth ; Texture: moist and easy to stir
Conclusion:	good viability, no abnormal colony morphology, qualified

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