Tengcang Fusarium|187705 |BNCC

Essential Information Certificate Related Products

Gibberella fujikuroi

Subculture procedure	① Dissolve the Frozen vials in a water bath (fungi at 30 ℃, bacteria at 37 ℃) and shake rapidly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μ L per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	28 ℃; 3-5 days; aerobic;
Storage conditions	-196 ℃
Safety level	1
morphology	The front is white, with a colony texture of fluff or cotton; The surface is flat and slightly raised
Sharing mode	Public welfare sharing

Gibberella fujikuroi

Storage conditions: 2~8 ℃

No. 187705

Product format: agar slant in 14mm test tube

Validity: growing culture, in 30 days

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Gorwth conditions: 28 ℃, aerobic, integrated PDA,3-5 days.
Comprehensive PDA: Potato boiling liquid 1.0L, glucose 20.0g, KH2PO4 3.0g, MgSO4 7H2O 1.5g, vitamin B1 trace amount, agar 20.0g, pH 6.0±0.2. Sterilize at 121℃ for 15min.

Potato boiling liquid : weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0l.

Receiving notice: If any abnormality is found on the day of receipt, please contact customer service in time within 24 hours. The tube bevel can be used directly. In principle, it can be used many times without stain during the storage period, but the vitality will gradually decrease with time. Please operate in strict accordance with the instructions. Otherwise, the replacement service will not be provided if abnormal strains and inactivation are caused.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm²;

(4) lay flat the small pieces to the center of the agar plate;

(5)put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 4days strain layer become obvious
colony morphology:	small filamentous fungi with obvious colonies on comprehensive PDA medium, white, hyphae are dense and vigorous, spreading to the edge of the plate, and the back of the medium is light yellow.
Conclusion:	good viability, no abnormal colony morphology, qualified

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