Lactobacillus acidophilus|196182 |BNCC

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Ligilactobacillus acidipiscis

Subculture procedure	① Prepare 1-2 plates as above (pre-deoxygenate in anaerobic environment for 24h); ② Open in biosafety cabinet, cauterize top with alcohol lamp, then rapidly break with sterile water; crush with forceps; ③ Pipette 0.5mL sterile water into freeze-dried vial, dissolve powder completely, then dispense 200μL/Agar plate, spread evenly; ④ Incubate plates under specified conditions until colonies emerge.
Growth conditions	37°C, 24-48h, microaerophilic
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 0.5–1 mm; circular, opaque; upper surface milky white with central elevation; smooth texture; moist; non-pigmented; Gram-positive (blue-violet); bacillus; purity: pure
Sharing mode	Public welfare sharing

1.Description

1.Name：Lactobacillus acidipiscis

2. BNCC No.：196182
3. Biosafety level：1
2.Storage conditions：
Storage of freezed dried ampoule and agar slant at 2°C to 8°C
3.Growth Conditions
1. MRS medium: cheese peptone 10.0g, beef powder 8.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.05g, Tween 80 1.0g, distilled water 1000mL. pH6.2±0.2. 121 ℃,15min.
2. Atmosphere： micro aerobic

3. Temperature：37 ℃

4.Notes：

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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