Mushroom|336091 |BNCC

Essential Information Certificate Related Products

Lentinus edodes (Berkeley) Singer

Subculture procedure	① Prepare 1-2 plates as mentioned above; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the Agar plate and onto the surface of the agar; ⑤ Cultivate the flat Agar plate under the above cultivation conditions, and the strain can be used once it grows.
Growth conditions	28 ℃; 7-10 days; aerobic;
Storage conditions	Liquid nitrogen
Safety level	1
morphology	Small filamentous fungi, cultured on a comprehensive PDA medium, have low and flat colonies with white hyphae that spread and grow towards the edge of the agar plate
Sharing mode	Public welfare sharing

Lentinus edodes

Storage conditions: 2~8 ℃

No.: 336091

Product format: agar slant in 14mm test tube

Validity period: 30 days

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Growth conditions:25-28 ℃, aerobic, comprehensive PDA. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH2PO4 3g, MgSO4.7H2O 1.5g, thiamine trace, agar 15g,pH natural.

Recovery steps:
(1)Prepare 1-2 pieces of PDA plates;
(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;
(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;
(4)Lay flat the small pieces to the center of the agar plate;
(5)Put the plates under the above culture conditions for cultivation for 5-7days. Or contact our technicians for the inoculation of agar slant - filamentous fungi.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 7 days strain layer is obvious
colony morphology	small filamentous fungi, colonies are obviously visible on comprehensive PDA medium, hyphae are white and dense, and often split into teeth.
Conclusion	good viability, no abnormal colony morphology, completely consistent with the above figure, qualified

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