Basidiomycetes|341554 |BNCC

Essential Information Certificate Related Products

Talaromyces sp.

Subculture procedure	① Prepare 1-2 plates as described above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then promptly add sterile water to rupture; break open with forceps; ③ Pipette 0.5mL sterile water into freeze-dried tube, dissolve powder completely, then dispense 200μL/Agar plate and spread evenly; ④ Incubate plates under specified conditions until colonies appear, then the culture is ready for use.
Growth conditions	28°C; 5-7 days; aerobic;
Storage conditions	2-8°C
Safety level	1
morphology	Small filamentous fungus; colonies are clearly visible on malt extract agar medium. Initial mycelium is white, dense, and vigorous, later turning bluish-gray with wrinkles on the underside of the medium
Sharing mode	Public welfare sharing

Penicillium funiculosum

Storage conditions: 2~8 ℃

No. 341554

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :28 ℃, aerobic, wort agar, 5-7 days, wort agar: malt extract 20.0g, glucose 20.0g, peptone 1.0g, agar 20.0g, distilled water 1.0L,pH natural. Sterilization at 121 ℃ for 15min.

Recovery steps:
(1) Prepare 1-2 of above mentioned plates;
(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions for cultivation for 5-7 days.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability,in 7 days plate colonies are obvious
colony morphology: (above)	small filamentous fungi, the colony is obvious on the wort agar medium, the initial hyphae are white, dense and vigorous, and the gray spores are full of plates.
Conclusion:	good viability, no abnormal colony morphology, qualified

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