BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC352246 |
| Description | R2A Agar |
| Composition | Peptone:0.5g, Casein Hydrolysate:0.5g, Yeast Extract:0.5g, Soluble Starch:0.5g, K2HPO4:0.3g, MgSO4:0.024g, Sodium Pyruvate(C3H3NaO3):0.3g, Agar:15.0g, Glucose:0.5g, pH:7.2±0.2(25℃) |
| Growth conditions | 28 ℃; 24-48 hours; aerobic |
| Subculture procedure | ① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 0.5-1mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth on the surface, moist texture, easy to pick up, G+(blue purple), Bacillus subtilis |
| Sharing mode | Public welfare sharing |
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