Clostridium knowlesiana|368424 |BNCC

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Clostridium novyi

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 48-72h; anaerobic;
Subculture procedure	① Dissolve the frozen tube in a 37 ℃ water bath and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to the anaerobic workstation for operation; ③ Unscrew the tube cap, extract all the dissolved solution, and transfer it into 1-2 agar plates (for anaerobic bacteria cultivation, the culture medium should be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation. ⑤ The anaerobic cultivation environment can also be carried out in a 350ml anaerobic cultivation bag containing anaerobic gas generator.
Storage conditions	-80 ℃
Safety level	2
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white on the front (hemolysis), raised in the middle, smooth on the surface, moist texture, easy to pick up, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing