BeNa Culture Collection
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| Culture medium | BNCC352250 |
| Description | Comprehensive PDA agar |
| Composition | Potato Infusion Powder:10.0g, Glucose:20.0g, KH2PO4:3.0g, MgSO4.7H2O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃) |
| Growth conditions | 28°C; 7-10 days; aerobic |
| Subculture procedure | ① Prepare 1-2 plates as described above; ② Disinfect the surface of the test tube slants before opening them in a biosafety cabinet; ③ Cut blocks (see appendix) into 0.5×0.5 cm² squares using a sterile inoculation fork and inoculation spatula; ④ Place the small square blocks flat onto the center of the Agar plate, on the agar surface; ⑤ Incubate the plates upright under the above conditions until the strain grows, then it is ready for use. |
| Storage conditions | Liquid nitrogen |
| Safety level | 1 |
| morphology | Spreading growth, filamentous, irregular edges, opaque, white upper surface, rough texture, dry consistency |
| Sharing mode | Public welfare sharing |
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