Pseudomonas aeruginosa|133928 |BNCC

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Pseudomonas azotoformans

Subculture procedure	① Prepare 1-2 plates as above; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water into the lyophilized vial, dissolve the powder completely, then dispense 200μL/well onto plates, spreading evenly; ④ Incubate plates under the specified conditions; the strain is ready for use once growth appears.
Growth conditions	30°C; 18-24h; aerobic
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 0.5–1 mm; circular, with neat margins; opaque, yellowish surface; pale color; central elevation; smooth, glossy surface; moist texture; easily liftable; G− (red); bacillus
Sharing mode	Public welfare sharing

Pseudomonas azotoformans

Storage conditions: 2~8 ℃

No. : 133928

Product format : freeze dried,200ul

Validity period: Freeze-dried tube for 24 months

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery/subculture :30°C, aerobic, nutrient gravy medium (NB). Nutritional gravy medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, distilled water 1.0L, pH7.0. 121 ℃,15min.

Recovery steps:

(1)Prepare a flask of NB liquid media.

(2)Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 30℃ (140r/min) for 18-24 hours;

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 20h NB bacterial fluid become turbid; The bacterial liquid is marked with NA plate, and the colony is obvious
colony morphology	size: medium color: yellowish shape: round edge: neat edge dry and wet: wet and smooth: smooth transparency: opaque uplift: bulge
Conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified.

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