Bifidobacterium longum|186131 |BNCC

Essential Information Related Products

Bifidobacterium longum

Culture medium	BNCC360803
Description	Enhanced Clostridium agar medium
Composition	Yeast Extract:3.0g, Beef Extract Powder:10.0g, Glucose:5.0g, Pancreatic Casein Peptone:10.0g, NaCl:5.0g, Soluble Starch:1.0g, L-Cysteine Hydrochloride(C₃H₇NO₂S.HCl):0.5g, CH₃COONa:3.0g, Agar:12.5g, pH:6.8±0.2(25℃)
Growth conditions	37°C; 24-48h; anaerobic;
Subculture procedure	① Prepare 1-2 plates as above (pre-deoxygenated in anaerobic environment for 24h); ② Open in a biosafety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it. Break it into pieces using forceps; ③ Pipette 0.5mL sterile water (pre-deoxygenated in anaerobic conditions for 24h) into the freeze-dried vial. After fully dissolving the bacterial powder, dispense 200μL/Agar plate and spread evenly; ④ Incubate plates under the specified conditions. The culture is ready for use once colonies appear.
Storage conditions	2-8℃
Safety level	1
morphology	Colony diameter: 1-2 mm; circular, with neat edges; opaque, creamy white on top; convex center; smooth surface; viscous texture; Gram-positive (blue-violet); bacillus
Sharing mode	Public welfare sharing