Triangular fungus|357690 |BNCC

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Microascus trigonosporus

Subculture procedure	① Prepare the above two tablets; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ° C; 5-7 days; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Small filamentous fungi grow well on malt extract agar medium, producing white hyphae on the front of the medium in the early stage and gray spores in the later stage. The back of the medium is dark brown with yellow edges
Sharing mode	Public welfare sharing

Microascus trigonosporus

Storage conditions : 2~8 ℃

No. : 357690

Product format : freeze dried,200ul

Validity period: 6 years

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :28 ℃, aerobic, wort agar, 5-7 days, wort agar: malt extract 20.0g, glucose 20.0g, peptone 1.0g, agar 20.0g, distilled water 1.0L,pH natural. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates；

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 5-7 days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 5-7 days plate colony is obvious
colony morphology (above)	small filamentous fungi have obvious colonies on the integrated PDA medium, the initial hyphae are white, dense and vigorous, and the gray spores are full of plates
conclusion	good viability, no abnormal colony morphology, qualified

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