Acidobacterium acidophilus|353574 |BNCC

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Bacteroides acidifaciens Miyamoto and Itoh 2000

Subculture procedure	① Prepare 2 plates (placed in anaerobic environment for 24 hours of deoxygenation in advance); ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water (placed in an anaerobic environment for 24 hours of deoxygenation in advance) and pour it into a freeze-drying tube. After fully dissolving, mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used.
Growth conditions	37 ℃; 48-72 hours; anaerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth on the surface, moist texture, easy to pick up, G - (red), Bacillus
Sharing mode	Public welfare sharing

Bacteroides acidifaciens

Storage conditions : 2~8 ℃

No. : 353574

Product format: freeze dried, 200ul

Validity : 6 years

iBosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :37 ℃, strictly anaerobic, Colombian blood plate (ready-to-use type),48-72h.

Recovery steps:
(1) Prepare 2 of above mentioned plates;
(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5ml of sterile water(keep in an anaerobic environment for 24 hours) into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions for cultivation for 48-72hours.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 48-72h strain layer become obvious, colony is typical
colony morphology (above)	Size: 1-2mm Shape: Round Edge: Neat Transparency: Translucent Color: off-white uplift: middle raised surface: bright and smooth texture: moist and viscous
Conclusion	good viability, no abnormal colony morphology, qualified

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