Copper greedy bacteria|356124 |BNCC

Essential Information Certificate Related Products

Cupriavidus sp.

Subculture procedure	① Dissolve the frozen tube in a water bath (37 ℃ for bacteria) and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates; ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	30 ℃; 18-24h; aerobic;
Storage conditions	-80 ℃
Safety level	1
morphology	Size: 1-2mm Shape: Circular Edge: Neat Transparency: Opaque Color: Grey White Rise: Middle Rise Surface: Smooth and Bright Texture: Wet and Thick
Sharing mode	Public welfare sharing

Cupriavidus sp.

Storage conditions: 2~8 ℃

No. 356124

Product format: 90mm flat plate

Validity period: 10 days

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 30℃, aerobic, nutrient agar medium, 18-24h. Nutrient agar medium: beef extract 3.0g, peptone 10.0g, NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L, pH 7.0. Sterilize at 121°C for 15min.

Recovery steps:

In a sterile environment, use a sterile inoculation ring to pick up a sufficient amount of plate colonies, transfer them to a plate or liquid culture medium, and culture them under suitable conditions for 18-24 hours. the bacteria can be used when they grow out.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability,in 24 hours,liquid medium become turbid, colony is typical on marked plate
colony morphology: (above)	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: off-white uplift: middle raised surface: smooth and bright texture: moist and viscous
Conclusion	good viability, no abnormal colony morphology, qualified

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