Cat ear slanted umbrella|357891 |BNCC

Essential Information Certificate Related Products

Clitopilus passeckerianus

Subculture procedure	① Dissolve the Frozen vial in a water bath (fungi at 30 ℃) and shake rapidly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates; ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	28 ° C; 5-7 days; aerobic;
Storage conditions	-80 ℃
Safety level	1
morphology	A small filamentous fungus that grows well on comprehensive PDA medium. The obverse side of the colony appears white with spreading growth, while the reverse side of the medium shows a yellow coloration.
Sharing mode	Public welfare sharing

Clitopilus passeckerianus

Storage conditions : 2~8 ℃

No.: 357891

Product format : freeze dried,200ul

Validity period: 6 years

Biosafety level : 1,handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28°C, aerobic, synthetic PDA, 5-7 days. Comprehensive PDA: Potato cooking liquid 1.0L, glucose 20.0g, KH2PO4 3.0g, MgSO4 7H2O 1.5g, trace amount of vitamin B1, agar 20.0g, pH 6.0±0.2. Sterilize at 121℃ for 15min. Potato cooking liquid: Weigh 200g of peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates（place in anaerobic environment for 24hours before usage）；

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 5-7 days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 7 days plate colony is obvious
colony morphology	small filamentous fungi have obvious colonies on the integrated PDA medium, with low and flat colonies, white, and light yellow on the back of the medium
conclusion	good viability, no abnormal colony morphology, qualified

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