Crystal ghost umbrella|120570 |BNCC

Essential Information Related Products

Coprinus micaceus (Bull.) Fr.

Culture medium	BNCC352250
Description	Comprehensive PDA agar
Composition	Potato Infusion Powder:10.0g, Glucose:20.0g, KH₂PO₄:3.0g, MgSO₄.7H₂O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃)
Growth conditions	25-28 ℃, 5-7 days, aerobic
Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the Agar plate and onto the surface of the agar; ⑤ Cultivate the flat Agar plate under the above cultivation conditions, and the strain can be used once it grows.
Storage conditions	Liquid nitrogen
Safety level	1
morphology	small filamentous fungus cultured on comprehensive PDA medium exhibits distinct colonies. The colonies are white, with dense and vigorous mycelium that spreads towards the edges of the Agar plate. The reverse side of the medium appears pale yellow.
Sharing mode	Public welfare sharing