Penicillium flavum|185782 |BNCC

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Penicillium chrysogenum

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	28 ℃; 3-5 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	On standard Potato Dextrose Agar (PDA), this small filamentous fungus forms distinct colonies. The mycelial mat undergoes a color change from initial white to yellow, culminating in the production of gray spores at maturity. The reverse side of the agar exhibits a pale yellow coloration.
Sharing mode	Public welfare sharing

Penicillium chrysogenum

Storage conditions: 2~8 ℃

No.185782

Product format: freeze dried,200ul

Validity: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The agar slant can be used directly. In principle, it can be used many times without contamination within the validity period, but viability will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28°C, aerobic, integrated PDA, 3-5 days. Comprehensive PDA: Potato cooking liquid 1.0L, glucose 20.0g, KH2PO4 3.0g, MgSO4 7H2O 1.5g, trace amount of vitamin B1, agar 20.0g, pH 6.0±0.2. Sterilize at 121℃ for 15min. Potato cooking liquid: Weigh 200g of peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:

①Prepare 1-2 of above mentioned plates;

②Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

③Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

④Put the plates under the above culture conditions for cultivation 3-5days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 4 days strain layer become obvious
colony morphology:	small filamentous fungi have obvious colonies on the comprehensive PDA medium. The mycelium of the plate layer is white in the early stage and gradually turns yellow, and gray spores are produced in the later stage, and the back of the medium is light yellow.
Conclusion:	good viability, no abnormal colony morphology, qualified

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