Clostridium sporogenes|104015 |BNCC

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Clostridium sporogenes

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 18-24h; Anaerobic
Subculture procedure	① Prepare two of the above-mentioned agar plates and sterile water (pre-equilibrated in an Anaerobic environment for 24 hours);② Disinfect the surface of the ampoule tube, open it in a biosafety cabinet, flame the top with an alcohol lamp, then quickly drip sterile water onto it to crack it open, and use tweezers to break it apart;③ Aspirate 0.5 mL of sterile water, inject it into the freeze-dried tube, fully dissolve the bacterial powder, then transfer it onto two agar plates, 200 μL per plate, and spread evenly;④ Place the plates under the aforementioned culture conditions for incubation. The bacterial strain can be used once growth is observed.
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony has a diameter of 0.5–1 mm, appearing circular with neat edges and an opaque texture. It is grayish-white on the obverse side, with a raised center, smooth surface, and moist consistency. The colony is easily picked up, tests Gram-positive (showing blue-violet staining), and consists of rod-shaped bacteria.
Sharing mode	Public welfare sharing