Bacillus thuringiensis|359181 |BNCC

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Cetobacterium somerae

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	37 ℃; 3-4 days; anaerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, semi transparent, dark color, smooth surface, bright surface, moist texture, does not produce pigments, G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing

Cetobacterium somerae

Storage conditions: 2~8 ℃

No. 359181

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Culture conditions:37 ℃, anaerobic, Colombian blood plate (ready-to-use type),3-4 days.

Recovery steps:

①Prepare 1-2 of above mentioned plates;

②Open in the the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

③Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

④Put the plates under the above culture conditions for cultivation，and the strain can be used when it grows out.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability，in 4 days obvious strain layer occurs on the plate; colony is typical
colony morphology: (above)	Size: 2-4mm Shape: Round Edge: Irregular Transparency: Opaque Color: White Protuberance: Flat Surface: Smooth Texture: Wet and Easy to Stir
conclusion: good viability, no abnormal colony morphology, qualified

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