BeNa Culture Collection
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| Culture medium | BNCC360873 |
| Description | LB Medium with 50μg/mL Streptomycin |
| Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25℃) |
| Growth conditions | 28 ℃; 24-48 hours; aerobic |
| Subculture procedure | ① Prepare 1-2 plates as described above.② In a biosafety cabinet, open the vial and briefly flame the top with an alcohol burner, then immediately add sterile water to break the seal. Use sterile forceps to crush the vial.③ Aspirate 0.5 mL of sterile water into the freeze‑dried vial, thoroughly dissolve the bacterial powder, then transfer 200 μL of the suspension onto each Agar plate and spread evenly.④ Incubate the plates under the specified culture conditions. The strain is ready for use once growth appears. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colonies measure 1-2 mm in diameter, are circular with regular margins, and are opaque. They exhibit a pale yellow pigmentation on the surface, have a raised center, and are smooth, glossy, and moist in texture. The organisms are Gram-negative (G⁻, appearing red) and rod-shaped (bacilli). Purity: pure. |
| Sharing mode | Public welfare sharing |
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