Legionella pneumophila subsp. pneumophila|362067 |BNCC

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Legionella pneumophila subsp. pneumophila

Culture medium	BNCC360928
Description	Buffered Charcoal Yeast Extract Agar
Composition	Yeast Extract Powder:10.0g, Activated Charcoal:2.0g, Agar:13.0g, α-Ketoglutarate Monopotassium Salt(C₅H₅KO₅):1.0g, ACES Buffer:10.0g, Potassium Hydroxide(KOH):2.8g, Ferric Pyrophosphate(Fe₄(P₂O₇)₃):0.25g
Growth conditions	37 ℃; 48-72 hours; 5% CO2
Subculture procedure	① Dissolve the frozen tube in a water bath (37 ℃ for bacteria) and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates; ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions	-80 ℃
Safety level	2
morphology	The colony has a diameter of 1-2mm, irregular shape, irregular edges, opaque, gray white on the front, raised in the middle, smooth surface, moist texture, easy to pick up, G - (red), rod-shaped
Sharing mode	Public welfare sharing