Ergot fungus|358174 |BNCC

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Claviceps purpurea

Subculture procedure	① Prepare one 90mm solid Agar plate, one inoculation shovel and one inoculation hoe; ② Inoculation shovel, inoculation hoe, flame burn, cool for later use; ③ After disinfecting the outer wall of the inclined surface, open it in a sterile environment, first use an inoculation hoe, and then use an inoculation shovel to cut into pieces; ④ Inoculate the bacterial block with a spatula, place it on the surface of a agar plate, and cover the Agar plate; ⑤ Place the flat Agar plate in the fungi incubator and cultivate it according to the above conditions. Do not seal it with a sealing film. The Agar plate can be used when it is almost full.
Growth conditions	28 ° C; 5-7 days; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Small filamentous fungi, with obvious colonies on comprehensive PDA medium, white, low and flat, and light yellow on the back of the medium
Sharing mode	Public welfare sharing

Claviceps purpurea

Storage conditions: 2~8 ℃

No. 358174

Product format: freeze dried,200ul

Validity: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Growth conditions: 28°C, aerobic, integrated PDA, 5-7 days. Comprehensive PDA: Potato cooking liquid 1.0L, glucose 20.0g, KH2PO4 3.0g, MgSO4 7H2O 1.5g, vitamin B1 trace, agar 20.0g, pH 6.0±0.2. Sterilize at 121℃ for 15min. Potato cooking liquid: Weigh 200g peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates；

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 5-7 days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 7 days plate colony become obvious
colony morphology: (above)	small filamentous fungi, with obvious colonies, white, low and flat, and light yellow on the back of the medium on the integrated PDA medium.
Conclusion	good viability, no abnormal colony morphology, qualified

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