BeNa Culture Collection
info@bncc.com
| Subculture procedure | ① Dissolve the Frozen vial in a water bath (37 ℃) and shake rapidly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates; ④ After uniform coating, transfer to the above cultivation conditions for cultivation. |
| Growth conditions | 37 ℃; 48-72h; slightly aerobic; |
| Storage conditions | -80 ℃ |
| Safety level | 2 |
| morphology | The colony diameter is 0.5-1mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth on the surface, moist texture, easy to pick up, G - (red), Bacillus |
| Sharing mode | Public welfare sharing |
Streptomyllum rosemicum
Storage conditions: 2~8 ℃
No. 359331
Product format: 90mm flat plate
Validity: growing culture, in 10 days
Biosafety level: 2, handle in safety cabinet
Receiving notice: if any abnormality is found on the day of receiving goods, please contact customer service within 24 hours in time. if it is overdue, it will be deemed as good receipt. Dry powder shall not be retained after one use, and it can be used after being activated for 1~2 generations. please refer to the attached page for the use of the flat panel. Please activate in strict accordance with this instruction, otherwise the replacement service will not be provided if the strain is abnormal and inactivated.
Growth conditions:37 ℃, slightly aerobic, Colombian blood plate (ready-to-use type),2-3 days.
Recovery steps:
① Prepare the above flat plate 1-2 block;
② Open the flat surface in the safety cabinet after disinfection;
③Use a sterile inoculation ring to pick the plate colony 1-2 ring and transfer it to a new medium;
④ Place the plate under the above culture conditions for 2-3 days.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:
| Item | test results |
| viability | good viability, in 2days strain layer is obvious;colony is typical on the streaked plate |
| colony morphology: (above) |
size: 0.5-1mm shape: round edge: irregular transparency: opaque color: white bulge: middle bulge surface: bright and smooth texture: moist and easy to stir |
| Conclusion | good viability, no abnormal colony morphology, qualified |