BeNa Culture Collection
info@bncc.com
| Subculture procedure | ① Dissolve the frozen tube in a 37 ℃ water bath and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to the anaerobic workstation for operation; ③ Unscrew the tube cap, extract all the dissolved solution, and transfer it into 1-2 agar plates (for anaerobic bacteria cultivation, the culture medium should be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation. ⑤ The anaerobic cultivation environment can also be carried out in a 350ml anaerobic cultivation bag containing anaerobic gas generator. |
| Growth conditions | 37 ℃; 48-72 hours; anaerobic |
| Storage conditions | -80 ℃ |
| Safety level | 2 |
| morphology | The colony has a diameter of 0.5-1mm, circular, opaque, gray white on the front, raised in the middle, smooth surface, moist texture, G - (red), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |