Bifidobacterium brevis|185357 |BNCC

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Bifidobacterium breve

Culture medium	BNCC360803
Description	Enhanced Clostridium agar medium
Composition	Yeast Extract:3.0g, Beef Extract Powder:10.0g, Glucose:5.0g, Pancreatic Casein Peptone:10.0g, NaCl:5.0g, Soluble Starch:1.0g, L-Cysteine Hydrochloride(C₃H₇NO₂S.HCl):0.5g, CH₃COONa:3.0g, Agar:12.5g, pH:6.8±0.2(25℃)
Growth conditions	37 ℃, 48-72 hours, anaerobic
Subculture procedure	① Prepare 1-2 fresh agar plates as specified above (pre-reduced in an anaerobic environment for 24 hours).② Within a biological safety cabinet, surface-sterilize and open the glass ampoule. Briefly flame the neck, then immediately apply a drop of sterile water to induce thermal cracking. Carefully open it using sterile forceps.③ Aspirate 0.5 mL of sterile, pre-deoxygenated water (similarly pre-reduced for 24 hours) and inject it into the freeze-dried vial. Mix thoroughly for complete resuspension. Transfer the entire suspension into a tube containing liquid medium and mix well.④ Inoculate 0.2 mL of the resulting bacterial suspension onto each pre-reduced agar plate and spread evenly. Prepare a duplicate Agar plate following the same method.⑤ Incubate all plates under the aforementioned anaerobic conditions. The culture is ready for use when visible growth appears.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colonies are 1-2 mm in diameter, circular with irregular margins, and opaque. The obverse side is creamy white, convex (raised at the center), with a smooth and moist texture. The cells are Gram-positive (staining blue-violet), rod-shaped. Purity: Pure.
Sharing mode	Public welfare sharing