BeNa Culture Collection
info@bncc.com
| Subculture procedure | ① Dissolve the frozen tube in a water bath (37 ℃ for bacteria) and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates; ④ After uniform coating, transfer to the above cultivation conditions for cultivation. |
| Growth conditions | 37 ℃; 48-72 hours; microaerophilic |
| Storage conditions | -80 ℃ |
| Safety level | 2 |
| morphology | The colony diameter is 0.5-1mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth on the surface, moist texture, easy to pick up, G - (red), Bacillus |
| Sharing mode | Public welfare sharing |
Campylobacter jejuni
Storage conditions: 2~8 ℃
No. 360736
Product format: freeze dried, 200ul
Validity period: 6 years
Biosafety level: 2, handle in safety cabinet
Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.
Recovery steps:
(1)Prepare 1-2 of above mentioned plates;
(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3)Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4)The plate is cultured under the above-mentioned culture conditions, and the strains can be used when they grow.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

| Item | test results |
| viability | good viability, in 72h strain layer become obvious, colony is typical |
| colony morphology: |
Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque color: white bulge: middle bulge surface: bright and smooth texture: moist and easy to stir |
| Conclusion: | good viability, no abnormal colony morphology, qualified |